猪圆环病毒2型对小鼠淋巴细胞发育的抑制作用

doi:10.11843/j.issn.0366-6964.2015.08.016

Abstract

Abstract:

Porcine circovirus type 2 (PCV2) is the pathogen of postweaning multisystemic wasting syndrome (PMWS)，and can reduce immune reaction and cause serious immunosupression by causing the apoptosis and depletion of lymphocyte directly.To investigate the influence of PCV2 on the early development of lymphocyte，we used mice as experiment model，then selected immunofluorescence assay to detece the CD3，CD19 positive cell number in bone marrow，thymus and spleen，selected qPCR to quantify the gene expression involved in lymphocyte development，and selected MTT assay to test the lymphocyte proliferation ability in vitro.The result showed that：1) PCV2 decreased of lymphocyte in peripheral blood during the early infection stage and inhibited the proliferation ability in vitro；2) PCV2 decreased the number of CD19 positive cell significantly (P<0.01) in bone marrow and spleen，some important gene which are involved in the development of lymphocyte were inhibited significantly；and 3) PCV2 promoted the apoptosis of lymphocyte in development stage.Taken together，the results presented here demonstrate that PCV2 inhibits the development of lymphocyte and induces lymphocyte apoptosis may be one of the reasons causing lymphocyte depletion in PMWS case.

CLC Number:

S852.659.2

WU Bin，XIAO Hui，HU Lin，YANG Liao-han，YE Shi-yi，HE Qi-gai，CAI Shuang-shuang，ZHANG Shu-jun. The Study on the Inhibition of Mouse Lymphocyte Development Caused by Porcine Circovirus Type 2[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(8): 1400-1408.

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The Study on the Inhibition of Mouse Lymphocyte Development Caused by Porcine Circovirus Type 2

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